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Figure 4: Complement activation by rFcnB/rMASP-2/rsMAP complexes. Upper panel: western blot of FcnB and MASP-2/sMAP. After incubation of the recombinant proteins as shown in the table, the generated complexes were pulled down by GlcNAc-agarose and subjected to western blotting. Lower panel: C4-deposition activity of the similar pull-down samples on GlcNAc-BSA-coated microplates. The samples used for C4 deposition were prepared the same as those for western blotting except for employment of rMASP-2a instead of rMASP-2i. The activity was determined in quadruplicate (mean ± SD).