Research Article

Mouse Ficolin B Has an Ability to Form Complexes with Mannose-Binding Lectin-Associated Serine Proteases and Activate Complement through the Lectin Pathway

Figure 5

Complex formation between rFcnB and rMASP-1i (a) and rMASP-3 (b). After incubation of the recombinant proteins as outlined in the table, samples pulled down with GlcNAc agarose were subjected to western blotting. Western blotting for rMASP-1i, rMASP-3, and rsMAP was performed using an anti-penta-His tag Ab.
105891.fig.005a
(a)
105891.fig.005b
(b)