A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants

Figure 6

WNV IgM capture ELISA with plant-derived DIII antigen and E16 mAbs. Columns 1 and 2 (positive controls): WNV IgM-positive serum (PC) detected by kit-supplied positive control antigen WNRA (1) or negative control antigen NCA (2) with the kit-supplied WNRA-specific antibody (Anti-WNRA); Columns 3 and 4 (negative controls): WNV IgM negative serum (NC) detected by WNRA (3) or NCA (4) with Anti-WNRA; Columns 5 and 6: PC detected by N. benthamiana-derived DIII antigen (B-DIII) (5) or NCA (6) with lettuce-derived E16 (L-E16); Columns 7 and 8: NC detected by B-DIII (7) or NCA (8) with L-E16; Columns 9 and 10: PC detected by B-DIII (9) or NCA (10) with N. benthamiana-derived E16 (B-E16); Columns 11 and 12: NC detected by B-DIII (11) or NCA (12) with B-E16.