Factors Affecting Poly(3-hydroxybutyrate) Production from Oil Palm Frond Juice by Cupriavidus necator (CCUG52238^T)

Table 2

Effect of agitation speed on the biosynthesis of P(3HB)^a.

Agitation speed (rpm)	CDW (g/L)	Total P(HB) (g/L)	P(3HB) content (wt.%)^b
180	7.37	1.62	22
200	8.30	2.66	32
220	9.42	3.77	40
240	6.37	1.72	27
260	5.19	1.09	21

^aMSM containing 16 g/L of total sugars in OPF juice and supplied with 1.0 g/L of (NH₄)₂SO₄, incubated at 30^°C for 48 h (initial pH medium adjusted at 7.0±0.1).
^bDetermination by GC from freeze dried samples.
^∗Values obtained herewith are means of two independent experiments.