Factors Affecting Poly(3-hydroxybutyrate) Production from Oil Palm Frond Juice by Cupriavidus necator (CCUG52238^T)

Table 3

Effect of (NH₄)₂SO₄ concentration on biosynthesis of P(3HB)^a_.

(NH4)2SO4 concentration (g/L)	CDW (g/L)	Total P(3HB) (g/L)	P(3HB) content (wt.%)^b
0.0	5.25	2.31	44
0.5	8.31	3.49	42
1.0	8.65	2.94	34
1.5	9.05	2.62	29
2.0	10.15	2.33	23

^aMSM containing 16 g/L of total sugars in OPF juice, incubated at 30°C for 48 h with agitation at 200 rpm (initial pH medium adjusted at 7.0±0.1).
^bDetermination by GC from freeze dried samples.
^∗Values obtained herewith are means of two independent experiments.