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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 131324, 6 pages
http://dx.doi.org/10.1155/2012/131324
Review Article

Canine Liver Transplantation Model and the Intermediate Filaments of the Cytoskeleton of the Hepatocytes

1Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, T6G 2B7, Canada
2Department of Laboratory Medicine and Pathology, Walter Mackenzie Health Sciences Centre, 5B4.09, 8440-112 Street, Edmonton, AB, T6G 2B7, Canada

Received 10 December 2011; Accepted 27 January 2012

Academic Editor: Andrea Vecchione

Copyright © 2012 Consolato Sergi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Liver transplantation has been a successful therapy for liver failure. However, a significant number of recipients suffer from graft dysfunction. Considerably, ischemia and reperfusion (I/R) injury is the most important factor leading to organ dysfunction, although the pathogenesis has not been fully described. I/R injury have several established features that are accompanied by and/or linked to bile duct loss or ductopenia, cholestasis, and biliary ductular proliferations in the posttransplant liver biopsy. However, biliary marker levels increase usually only 5–7 days after transplantation. Intermediate filaments are one of the three cytoskeletal proteins that have a major role in liver protection and maintaining both cellular structure and integrity of eukaryotic cells. We reviewed the canine liver transplantation model as I/R injury model to delineate the intermediate filaments of the cytoskeleton that are probably the determinants in changing the phenotype of hepatocytes to cholangiocytes. Remarkably, this interesting feature seems to occur earlier than frank cholestasis. We speculate that I/R liver injury through a phenotypical switch of the hepatocytes may contribute to the poor outcome of the liver graft.