136302.fig.002a
(a)
136302.fig.002b
(b)
136302.fig.002c
(c)
Figure 2: uPA binding to the cell surface in an integrin 𝛼 v 𝛽 3 -dependent and uPAR-independent manner [38]. (a) and (b) Depletion of uPAR from the cell surface blocked uPA binding to uPAR-CHO cells, but did not affect uPA binding to 𝛽 3 -CHO cells. To deplete GPI-linked uPAR on the cell surface, 𝛽 3 -CHO, uPAR-CHO, or control mock-transfected CHO cells were treated with PIPLC. The treatment removed more than 95% of human uPAR from uPAR-CHO cells as determined by flow cytometry with anti-uPAR mAb 3B10 (data not shown). uPA was immobilized to wells of 96-well microtiter plates at the indicated coating concentrations, and incubated with cells without (a) or with (b) pretreatment with PI-PLC. Bound cells were quantified. (c) uPA binding to 𝛽 3 -CHO cells is specific to 𝛼 v 𝛽 3 and the kringle domain. uPA (200 nM coating concentration) was immobilized to wells of 96-well microtiter plates and incubated with 𝛽 3 -CHO cells in the presence of mAb 16N7C2 (anti- 𝛽 3 ), Ab 963 (anti-kringle), mAb UNG-5 (anti-LMW-uPA), or RGD or RGE peptides (100  𝜇 M).