141395.fig.003a
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141395.fig.003b
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Figure 3: Alcohol-induced liver adiponectin receptors and involved genes in mRNA or protein expression. (a) The left panel shows the results of the RT-PCR analysis of adipoR1, adipoR2, SRIT1, PGC1α, and SREBP-1c expressions. The images show the representative agarose gel electrophoresis of the PCR products. The right panel shows the relative expressions of the adipoR1, adipoR2, SRIT1, PGC1α, and SREBP-1c levels. The data were normalized to the GAPDH levels, and all the expression levels that are shown are relative to the ND. (b) The upper panel shows the results of the Western blot analysis of the phosphorylated and total AMPK. The images show representative Western blotting images. The bottom panel shows the relative expressions of those levels and the ratios of the phosphorylated protein level to the total protein level. (c) The upper panel shows the results of the Western blot analysis of the phosphorylated and total ACC. The images show representative Western blotting images. The bottom panel shows the relative expressions of those levels and the ratios of the phosphorylated protein level to the total protein level. These results represent the means ± SEM of the three independent experiments in which RNA or protein samples from eight mice were used. a,b,cMeans not sharing a common letter are significantly different ( 𝑃 < 0 . 0 5 ).