Table 3: Description of selected recent diagnostic assessments of dengue NS1 RDTs.

AssayStudyYearLocationSample timingReference comparatorSensitivity (95% CI)Specificity (95% CI)

Dussart et al. [44]2008French Guiana82% <5 days illnessRT-PCR or paired MAC and GAC-ELISA77.6 (72.1–82.4)100 (92.6–100)
Shu et al. [42]2009TaiwanMedian 2; 1–7 days illnessRT-PCR or paired MAC and GAC-ELISA77.3 (0.54–0.92)100
Hang et al.[41]2009Vietnam1–6 days illnessRT-PCR or paired MAC and GAC-ELISA72.8 (64.1–80.3)100 (91.6–100)
Chaiyaratana et al. [43]2009Thailand1–8 days illnessNS1 Ag ELISA98.9 (96.8–100)90.6 (85.6–95.7)
Zainah et al. [45]2009MalaysiaNot statedViral culture, nested RT-PCR, NS1 Ag ELISA90.4 (86.6–94.4)99.5 (97.4–99.9)
Biorad STRIPRamierez et al. [46]2009Venezuela2–6 days illnessRT-PCR or paired MAC-ELISA67.8 (57.4–76.7)94.4 (80.9–99.4)
Lima et al. [47]2009Brazil1–6 days illnessCombinations of viral culture, nested RT-PCR, NS1 Ag ELISA89.6 (84.7–93.2)99.1 (96.9–99.9)
Pok et al. [38]2010Singapore1-8 days illness“Recife” classification (7)78.9 (70–86.1)99 (94.6–99.9)
Tricou et al. [39]2010Vietnam1–6 days illnessRT-PCR or paired MAC and GAC-ELISA61.6 (55.2–67.8)100 (93.8–100)
Najioullah et al. [37]2011MartiniqueNot statedRT-PCR49.4% (43.2–55.6)100
Osorio et al. [40]2010ColombiaMedian 4; range 2–7 days illnessViral culture, nested RT-PCR or paired MAC and GAC-ELISA57.7 (47.6–67.3)95.3 (84.2–99.4)
Blacksell et al. [19]2011Sri LankaMedian 5; IQR 2–7 days illnessAFRIMS MAC and GAC-ELISA paired samples58.6 (48.2–68.4)98.8 (95.6 −99.9)

Tricou et al. [39]2010Vietnam1–6 days illnessRT-PCR or paired MAC and GAC-ELISA62.4 (56.1–68.5)100 (93.8–100)
SD Bioline Dengue DuoWang and Sekaran [21]2010Malaysia1–15 daysVirus isolation, RT-PCR, paired MAC ELISA65.4 (58.5–72.3)98.8 (96.2–100)
Osorio et al. [40]2010ColombiaMedian 4; range 2–7 days illnessViral culture, nested RT-PCR or paired MAC and GAC-ELISA51 (44.1–57.7)96.7 (90.8–99.3)
Blacksell et al. [19]2011Sri LankaMedian 5; IQR 2–7 days illnessAFRIMS MAC and GAC-ELISA paired samples48.5 (38.5–58.7)99.4 (96.6–100)

Panbio Early Rapid NS1Fry et al. [20]2011Vietnam1-5 days; 84.5%<3 days illnessRT-PCR or paired MAC and GAC-ELISA69.2 (62.8–75.6)96% (92.2– 99.8)
Fry et al. [20]2011Malaysia1-15 days; 70% ≤5 days illnessRT-PCR or paired MAC and GAC-ELISA68.9 (61.8–76.1)96.7 (82.8– 99.9)
Blacksell et al. [19]2011Sri LankaMedian 5; IQR 2–7 days illnessAFRIMS MAC and GAC-ELISA paired samples58.6 (48.2–68.4)92.5 (87.3–96.1)