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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 161372, 7 pages
http://dx.doi.org/10.1155/2012/161372
Research Article

Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining

Key Laboratory of Animal Biotechnology of Xinjiang and Key Laboratory of Grass Livestock Reproduction and Breed Biotechnology, Ministry of Agriculture, Animal Science Academy of Xinjiang Uygur Autonomous Region, Urumqi 830000, China

Received 24 January 2012; Revised 28 February 2012; Accepted 22 March 2012

Academic Editor: Brad Upham

Copyright © 2012 Liqin Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies. Generally, determination of oocyte quality is mostly based on the layers of cumulus cells and cytoplasm or cytoplasm uniformity and colors. This requires considerable experience to better identify oocyte quality because of the intense subjectivity involved (Gordon (2003), Madison et al. (1992) and De Loos et al. (1992)). BCB staining is a function of glucose-6-phosphate dehydrogenase (G6PD) activity, an enzyme synthesized in developing oocytes, which decreases in activity with maturation. Therefore, unstained oocytes (BCB−) are high in G6PD activity, while the less mature oocytes stains are deep blue (BCB+) due to insuffcient G6PD activity to decolorize the BCB dye.