New Insights into p53 Signaling and Cancer Cell Response to DNA Damage: Implications for Cancer Therapy

Figure 5

Immunofluorescence analysis of p53 and p21 protein expression in A172 cells after exposure to ionizing radiation (IR; 5 Gy). (a) Representative images showing p53 and p21 protein levels in control (non-irradiated) and irradiated cells. [b] Percentages of cells exhibiting nuclear accumulation of p53 or p21 before (control) and at indicated times post-irradiation. Each datum point represents the mean (±SD) values of duplicate samples. At least 200 cells were evaluated for each sample. Immunofluorescence analysis of p53 and p21 protein levels was performed using our published techniques [18].