About this Journal Submit a Manuscript Table of Contents
Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 184538, 11 pages
http://dx.doi.org/10.1155/2012/184538
Research Article

Topical Application Effect of the Isolectin Hydrogel (Cramoll 1,4) on Second-Degree Burns: Experimental Model

1Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil
2Programa de Pós-Graduação em Biociência Animal, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil
3Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil
4Divisão de Radiofarmácia, Instituto de Engenharia Nuclear, 21941-906 Rio de Janeiro, RJ, Brazil
5Departamento de Patologia, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil
6Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil

Received 24 August 2011; Revised 31 October 2011; Accepted 8 November 2011

Academic Editor: Monica Fedele

Copyright © 2012 Danielle dos Santos Tavares Pereira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed at evaluating the use of hydrogel isolectin in the treatment of second-degree burns. Twenty male rats were randomly divided into two groups (G1 = treatment with hydrogel containing 100 μg/mL Cramoll 1,4 and G2 = Control, hydrogel). After 7, 14, 21, 28, and 35 days, animals were euthanized. On the 7th day, G1 showed intense exudates, necrosis and edema. On the 14th day, G1 showed tissue reepithelialization and moderate autolysis. On the 21st day, G1 showed intense fibroblastic proliferation, presence of dense collagen, and moderate fibrosis. On the 28th day, G1 showed complete tissue epithelialization. On the 35th day, G1 showed modeled dense collagen. The significant wound contraction was initiated from day, 14 in the G1. There were no significant differences in biochemical and hematological parameters analyzed. These results extend the potential of therapeutic applications for Cramoll 1,4 in the treatment of thermal burns.