Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants

Figure 1

Approaches to BAC library screening. Genetically mapped markers and unmapped sequence information such as cDNA and ETSs can be used to screen multidimensional BAC pools via PCR-based methods, hybridization of BAC filters, or highly parallel array platforms. The resulted data are deconvoluted to obtain marker BAC relationships prior to integrating to the contig map. Alternatively the BAC sequence information obtained through survey sequencing (NGS/Sanger sequencing) or BES could be searched against genetically mapped markers, ESTs, cDNA, and so forth of the same organism or different species.