186710.fig.003a
(a)
186710.fig.003b
(b)
Figure 3: Plasmin elicits CCL20 protein expression in dendritic cells. (a) Plasmin induces a concentration-dependent release of CCL20. Dendritic cells were stimulated with various concentrations of plasmin or LPS (0.5 μg/mL) for 24 h before being analyzed by ELISA. The results are mean ± SEM of 8 experiments, *P < 0.05, **P < 0.01 versus control. (b) Flow cytometric analysis of CCL20 expression by dendritic cells. Dendritic cells were either unstimulated or stimulated with plasmin (0.143 CTA U/mL) for 24 h. Brefeldin A was added to the cell culture 4 h before the end of the incubation, and the cells were fixed, permeabilized, stained, and analyzed by flow cytometry. Representative data of 3 independent experiments are shown.