206463.fig.003a
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206463.fig.003b
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206463.fig.003c
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206463.fig.003d
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206463.fig.003e
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206463.fig.003f
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Figure 3: The cell imaging and fluorescence intensity reflecting the interactions between soluble synthetic LeX and LeY determinants with DC-SIGN receptor on THP-1 leukemia cells, in the immunofluorescence assay. Control THP-1 cells, nondifferentiated (a) or differentiated (b) with PMA, GM-CSF and IL-4 stained with FITC-conjugated sheep antibodies against mouse Igs (secondary Ab). Nondifferentiated (c) and differentiated (d) THP-1 cells stained with mouse anti-DC-SIGN monoclonal antibodies (mAb) and with FITC-conjugated secondary Ab. Differentiated THP-1 cells preincubated with synthetic LeX (e) or LeY (f) determinants before the staining of the cells with anti- DC-SIGN mAb followed by the treatment with FITC-conjugated secondary Ab.