208108.fig.001
Figure 1: Characteristics of tPA-GFP expressing EA hy926 cells. (a) Immunofluorescence images of tPA-GFP expressing EA hy926 cells were captured by either epifluorescence microscopy (epi-F) or total internal reflection fluorescence microscopy (TIRF), the latter of which more clearly detects tPA-GFP containing granules as dots. (b) Culture media of tPA-GFP expressing EA hy926 cells and control cells were analyzed by western blotting. Additional high-molecular-weight bands detected by both anti-tPA and anti-GFP were observed in tPA-GFP expressing EA hy926 cells (orange arrows), suggesting that tPA-GFP has activity to form high-molecular-weight complex with PAI-1. (c) Fibrin autography of the same culture media. The additional bands detected by western blot analysis showed a lytic band (orange arrow). This figure was originally published in [3].