|
| PGM | MiSeq |
|
| Output | 10 MB–100 MB | 120 MB–1.5 GB |
| Read length | ~200 bp | Up to 2 × 150 bp |
| Sequencing time |
2 hours for 1 × 200 bp | 3 hours for 1 × 36 single read |
| 27 hours for 2 × 150 bp pair end read |
| Sample preparation time | 8 samples in parallel, less than 6 hrs | As fast as 2 hrs, with 15 minutes hand on time |
| Sequencing method | semiconductor technology with a simple
sequencing chemistry | Sequencing by synthesis (SBS) |
| Potential for development | Various parameters
(read length, cycle time, accuracy, etc.) | Limited factors, major concentrate in flowcell surface size, insert sizes, and how to pack cluster in tighter |
| Input amount | μg | Ng (Nextera) |
| Data analysis | Off instrument | On instrument |
|