Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells
Figure 1
Production of single chain protease NSB2- -NS3pro in E. coli. (a) The recombinant NS2B-NS3pro was produced as soluble proteins and purified by nickel column. L1: cell lysate, L2: washing flow through, L3: elution 1, and L4: elution 2. (b) Further purification was applied using gel affinity chromatography (95% of enzyme purity) L1 to L4: Elution 1 to 4. The activity of purified enzyme was assessed by catalyzing the fluorogenic peptide substrate (Boc-Gly-Arg-Arg-MCA).
