Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells

Figure 2

Dengue protease inhibition assay. The reaction mixtures consisted of fluorogenic peptide substrate and NS2B-NS3pro, with or without PG-1 of varying concentrations. The PG-1 was initially prepared in Tris-HCl buffer and assayed at five different concentrations, 10–160 μM. The best inhibition with low concentration of PG1 was observed at 40 μM with 95.7% inhibition.