Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells

Figure 3

Kinetic assay plot for the inhibition of the NS2B-NS3pro from dengue virus by PG-1 peptide. This assay was carried out using increasing concentration of inhibitor while all other conditions were kept constant. The data was analysed by Michaelis-Menten model under nonlinear regression curve fit in GraphPad Prism 5 software. The peptide concentrations were 0, 2.5, 5, 10, and 20 μM. The maximum enzyme velocity decreased from approximately threefold when PG-1 concentration was 20 μM.