Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells
Figure 4
Maximum nontoxic dose (MNTD) of PG-1.The MK2 cell lines were seeded at 1 × 104 cells per well in triplicate at optimal conditions (37°C, 5% CO2 in humidified incubator) in 96 well plates. PG-1 was diluted to serial concentrations 2.5, 12.5, 25, 50, 100, and 200 μM with DMEM media supplemented with 2% FBS. The cell culture was analyzed at 24, 48, and 72 hrs using Non-Radioactive Cell Proliferation assay (Promega, USA) according to the manufacture protocol. The toxic dose of MK2 cells was identified to be greater than 12.5 μM.
