Attenuated Salmonella typhimurium SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens

Table 1

Primers used for amplification of HA, NA, and NP of A/Ck/Malaysia/5858/04 (H5N1) in pcDNA3.1 vectors.

Primer	Sequence (5′ to 3′)	Gene	Tm (°C)
HAF	ccc caa GCT TAT GGA GAA AAT AGT GCT T	HA	58.5
HAR	ccc gga GGA TCC AAT GCA AAT TCT GCA TTG TAA	HA	63.2
NAF	ccc caa CAA GCT TAT GAA TCC AAA TAA GAA	NA	57.5
NAR	ccc gaa TTC CTT GTC AAT GGT GAA TGG	NA	59.7
NPF	aaa aag CTT ATG GCG TCT CAA GGC ACC	NP	59.7
NPR	ggg gga TCC ATT GTC ATA TTC CTC TGC	NP	61.3

Extra six nucleotide bases in lower case were added before recognition sites to facilitate amplification.