Research Article

Attenuated Salmonella typhimurium SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens

Table 2

Specific labeled probes used for the detection of AIV genes.

Sequence (5′ to 3′) Gene

Cy5 atg GAG AAA ATA GTG CTT CTT TTT GCA ATAHA
Alexa 350 atg AAT CCA AAT AAG AAG ATA ATA ACC ATCNA
Fluo atg GCG TCT CAA GGC ACC AAA CGA TCT TATNP

To facilitate binding of probe and labeling with dye, an additional 3 bp (atg) were added to the 5′ end of the primer.