PEDF and VEGF-A Output from Human Retinal Pigment Epithelial Cells Grown on Novel Microcarriers

Figure 3

VEGF-A concentration time course. The VEGF-A concentration in the media from the hRPE cells on the different microcarriers was measured with an ELISA. After a rising phase in the first 20 days after seeding the VEGF-A, concentration reached a plateau and remained stable until the end of the experiment. The hRPE cells on Hillex II, Plastic Plus, and ProNectin F microcarriers secreted comparable amount of VEGF-A, whereas hRPE cells on the CultiSpher microcarriers did not secrete significant amounts of VEGF-A.