Research Article

PEDF and VEGF-A Output from Human Retinal Pigment Epithelial Cells Grown on Novel Microcarriers

Figure 5

Regulation of VEGF-A expression in hRPE cells. Treatment of hRPE monolayers, grown in 24-well plates, with PTU (a tyrosinase blocker used to eliminate endogenous L-DOPA production) and 1 μM L-DOPA leads to a decrease in VEGF-A concentration in the medium. After washout of PTU and L-DOPA, the VEGF-A concentration returns to the baseline levels. Data are presented as the mean of three experiments conducted in triplicate, error bars represent S.E.M., and an asterisk (*) denotes 𝑃 < 0 . 0 0 0 5 using paired t-tests with Bonferroni’s correction between the PTU + L-DOPA group from both the baseline and the washout groups.
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