Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme
Figure 5
Alkaline phosphatase staining of cells grown in a DMEM/F12 culture medium with added follicular fluid (passage 3, day 44). (a, b) Some attached cells with a phenotype comparable to mesenchymal stem cells and small round cells with diameters of up to 5 μm (arrow), weakly positive for alkaline phosphatase activity. (c) Negative control. (inverted microscope, Hoffman). Scale Bar: 10 μm.
