In Vivo Clearance of Alpha-1 Acid Glycoprotein Is Influenced by the Extent of Its N-Linked Glycosylation and by Its Interaction with the Vessel Wall
Figure 2
Forms of AGP with reduced or absent N-linked glycosylation are cleared from the rabbit circulation more rapidly than native AGP following intravenous injection. (a) Shows a Coomassie blue-stained 12% SDS reducing gel loaded with 5–10 μg per lane of human serum albumin, HSA; AGP; AGP treated with PNGase F (PNGase) in the presence of either urea (PNGase-AGP (U) or SDS (PNGase-AGP (S); or recombinant yeast-derived nonglycosylated AGP (rAGP N(5)Q). (b) Depicts the residual acid-precipitable radioactivity remaining in the plasma of rabbits after intravenous injection of the indicated radiolabeled proteins as a function of time; for each protein, plasma radioactivity is presented as the percentage of the injected radioactive dose recovered in plasma at the indicated time after injection. Each point is the mean ± SD of 6 determinations; in most instances error bars are smaller than the symbol that denotes the mean.
