The Potential of Cellulosic Ethanol Production from Grasses in Thailand

Table 3

Ethanol production from grasses by SSCF process.

Grasses	Ethanol		% of the theoretical values
	g/L	g/g substrate
Sri Lanka	1.14 ± 0.09^a	0.14 ± 0.01^a	32.72 ± 2.69^a
Ratchaburi	1.10 ± 0.10^a	0.14 ± 0.01^a	30.95 ± 2.81^a,b
α-cellulose + xylan	1.06 ± 0.30^a,b	0.13 ± 0.04^a,b	23.01 ± 6.47^b
Dwarf napier	0.98 ± 0.16^a,b	0.12 ± 0.02^a,b	30.60 ± 4.90^a,b
Napier	0.97 ± 0.31^a,b	0.12 ± 0.04^a,b	30.30 ± 9.78^a,b
Bana	0.87 ± 0.14^a,b	0.11 ± 0.02^a,b	27.28 ± 4.56^a,b
Prachuap Khiri Khan	0.80 ± 0.12^b	0.10 ± 0.01^b	23.31 ± 3.48^b
King napier	0.40 ± 0.13^c	0.05 ± 0.02^c	13.93 ± 4.53^c
Atratum	0.36 ± 0.05^c	0.05 ± 0.01^c	11.64 ± 1.60^c
Ruzi	0.30 ± 0.06^c	0.04 ± 0.01^c	9.56 ± 1.79^c
Pangola	0.21 ± 0.05^c	0.03 ± 0.01^c	6.80 ± 1.58^c
Purple guinea	0.18 ± 0.07^c	0.02 ± 0.01^c	6.08 ± 2.49^c

Grasses (1.2 g) were pretreated with alkaline peroxide (7.5% (v/v) H₂O₂; pH 11.5; 35^°C, 24 h). SSCF process was performed using cellulase and xylanase (72 and 1440 U, resp.) from T. reesei for hydrolysis and S. cerevisiae and P. stipitis for cofermentation at 35^°C for 7 days. Positive control was 0.6 g of α-cellulose mixed with 0.6 g of xylan. Values not sharing a common superscript (a, b, c, and d) differ significantly (Duncan’s Multiple Range Test).