Quantitative Proteomic (iTRAQ) Analysis of 1st Trimester Maternal Plasma Samples in Pregnancies at Risk for Preeclampsia
Figure 1
Workflow for quantitative proteomics using iTRAQ reagent. Equal amounts of plasma protein (100 μg) from control and PE () were pooled separately and duplicated (technical replicates), controls were labeled with 114 and 116 iTRAQ label, and PE were labeled with 115 and 117 iTRAQ label. The labeled samples were pooled and were subjected to a strong cation exchange chromatography to remove the excess label. Afterwards, LC-MALDI MS/MS was performed for protein identification and quantification.
