Research Article

A New Candidate Substrate for Cell-Matrix Adhesion Study: The Acellular Human Amniotic Matrix

Figure 4

((a)–(c)) Cell colony formation on the AHAM for different cell types. (a) Bovine bone marrow stromal cells, (b) NIH 3T3 cells, and (c) HFFs were observed to form colonies as star-shaped cell clusters. Arrowheads indicate cell clusters, and arrows indicate extensions from the cell clusters. Scale bar, 100 μm. ((d)–(f)) Cells were observed to invade into the AHAM, as indicated by HE staining after 14 days of culture. (d) Bovine bone marrow stromal cells, (e) NIH 3T3 cells, and (f) HFFs. Arrows indicate cell clusters, and arrowheads indicate cells that are nearly penetrating the AHAM. Scale bar, 25 μm.
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