Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line
Figure 2
Inhibition of DC-SIGN expression by specific inhibitors of signaling pathways. DC-SIGN expression was detected on THP-1 cells differentiated by PMA, PMA plus IL-4, or PMA plus IL-4 treated with specific inhibitors (Helenalin, AG490, SB202190, and PD98059). (a), The relative levels of DC-SIGN mRNA detected by real-time PCR. The levels of DC-SIGN mRNA in PMA plus IL-4 treated THP-1 cells were valued as 100%, and used as a reference. *means ; **means . (b), DC-SIGN expression on surface of THP-1 cells detected by flow cytometry. The percentage of positive cells (top number) and mean fluorescence intensity (number below) are shown.
