Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line

Table 1

The sequence of the primers for DC-SIGN, the promoters, and internal reference.

	Sequence of the primers
P1 (the forward primer of DC-SIGN promoter)	5′-ATCATACGCGTATGAGTCCTTCTCCCTGTC-3′
P2 (the reverse primer of DC-SIGN promoter)	5′-TGTAAGATCTGTCACCCCACTCTCCCCCAG-3′
P3 (5′ side primer of AP-1 site)	5′-CAAAAGACTAGTGGAAAGCATCAGA-3′
P4 (3′ side primer of AP-1 site)	5′-CCCTACCCAACTTCCTGTTTCTCTT-3′
P5 (joint primer beside AP-1 site)	5′-CCACTAGTCTTTTGCCCTACCCAACTT-3′
P6 (5′side primer of Ets-1 site)	5′-TGGGTAGGGATCTGTCATCCA-3′
P7 (3′ side primer of Ets-1 site)	5′-TCTCTTTCTGTGGGAGACTAGAT-3′
P8 (joint primer beside Ets-1 site)	5′-CCACAGAAAGAGATGGGTAGGGATCT-3′
P9 (the forward primer of DC-SIGN)	5′-AGAGTGGGGTGACATGAGTGAC-3′
P10 (the reverse primer of DC-SIGN)	5′-GTGAAGTTCTGCTACGCAGGAG-3′
P11 (the forward primer of β-Actin)	5′-CTCCATCCTGGCCTCGCTGT-3′
P12 (the reverse primer of β-Actin)	5′-GCTGTCACCTTCACCGTTCC-3′

ACGCGT: the restriction sites of MLu I; AGATCT: the restriction sites of Bgl II.