Use of Recombination-Mediated Genetic Engineering for Construction of Rescue Human Cytomegalovirus Bacterial Artificial Chromosome Clones

Figure 6

1% Agarose gel electrophoresis of ORF targeted amplification reactions in WT Toledo BAC (WT), Region IV Deletion Mutant BAC (IV-Δ), and Region IV Rescued BAC (IV-R). Lane 1: 1 kb Plus DNA ladder. Lanes 2–4: amplification of UL 132 (~850 bases) with UL 132 F and R primers. Lanes 5–7: amplification of UL 151 (~1100 kb) with UL 151 F and R primers. Lanes 8–10: amplification of UL 148 (~1100 bases) with UL 148 F and R primers (positive control outside of region for deletion).