Figure 3: Endogenous neurogenesis induced by transplantation. Endogenous stem cells were assessed quantitatively by double staining of BrdU with nestin, GFAP, and NG2 at 1 and 2 weeks after transplantation in both the ependymal and parenchymal regions. (a)–(d) At 1 and 2 weeks following transplantation, BrdU/nestin-labeled cells as well as BrdU/GFAP-labeled astrocytes were present in ependyma. (e) and (h) BrdU-labeled NG2 cells were coexpressed at 1 and 2 weeks in the parenchyma. (i) and (j) The numbers of BrdU-labeled ependyma coexpressing GFAP/nestin were quantified at 1 and 2 weeks after transplantation. (k) The numbers of BrdU-labeled parenchyma coexpressing NG2 were quantified at 1 and 2 weeks after transplantation. , scale bars = 10 μm in (a)–(d); 20 μm in (e)–(h).