Evaluation of DNA Single and Double Strand Breaks in Women with Cervical Neoplasia Based on Alkaline and Neutral Comet Assay Techniques

Figure 2

Epithelial cells of the cervix processed using DBD-FISH on neutral comet to determine dsb and ssb simultaneously using whole-genome-labeled (red) and DAPI counterstained (blue) cells. Cell of patients with HG-SIL (c) and exhibit an increase in the migration length (dsb) and in the fluorescence intensity (ssb) compared with cells from control women ((a) and (b)). Apoptotic cell was concluded to form comets with large fan-like tails and small heads (i.e., so-called hedgehogs) (d).