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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 398398, 6 pages
http://dx.doi.org/10.1155/2012/398398
Research Article

Expression and Localization of Ryanodine Receptors in the Frog Semicircular Canal

1Department of Physiology, Section of General Physiology, University of Pavia, 27100 Pavia, Italy
2Brain Connectivity Center, IRCCS Foundation National Neurological Institute C. Mondino, 27100 Pavia, Italy
3Department of Physiology, Section of Human Physiology, University of Pavia, 27100 Pavia, Italy

Received 1 July 2011; Revised 5 October 2011; Accepted 11 October 2011

Academic Editor: Vickram Ramkumar

Copyright © 2012 Paola Perin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Several experiments suggest an important role for store-released Ca2+ in hair cell organs: drugs targeting IP3 and ryanodine (RyRs) receptors affect release from hair cells, and stores are thought to be involved in vesicle recycling at ribbon synapses. In this work we investigated the semicircular canal distribution of RyRs by immunofluorescence, using slice preparations of the sensory epithelium (to distinguish cell types) and flat mounts of the simpler nonsensory regions. RyRs were present in hair cells, mostly in supranuclear spots, but not in supporting cells; as regards nonsensory regions, they were also localized in dark cells and cells from the ductus. No labeling was found in nerve terminals, although nerve branches could be observed in proximity to hair cell RyR spots. The differential expression of RyR isoforms was studied by RT-PCR and immunoblotting, showing the presence of RyRα in both ampulla and canal arm and RyRβ in the ampulla only.