Table 1: List of SYBR Green screening methods developed and validated at WIV-ISP.

Method name Target Fragment size (bp) Reference RBCL Ribulose-1,5-biphosphate carboxylase oxygenase 95 [73] Plant taxon-specific methods LEC Lectin gene of soybean (Glycine max L.) 81 [74] ADH Alcohol dehydrogenase I gene from maize (Zea mays L.) 83 [73] CRU Cruciferin gene from oilseed rape (Brassica napus) 85 [73] PLD Phospholipase D gene from rice (Oryza sativa) 80 [73] SAD1 Stearoyl-acyl carrier protein desaturase gene of cotton (Gossypium genus) 107 [75] GLU3 Glutamine synthetase gene from sugar beet (Beta vulgaris) 118 [31] Generic element-specific methods p35S Promoter of the 35 S cauliflower mosaic virus 75 [28] tNOS Terminator of the nopaline synthase gene 69 [28] pFMV Promoter of the figworth mosaic virus 79 [76] pNOS Promoter of the nopaline synthase gene 75 [76] t35S Terminator of the cauliflower mosaic virus 107 in-house GM element-specific methods cryIAb/Ac Gene encoding the Bacillus thuringiensis δ-endotoxin (insect resistance) 73 [68] cry3Bb Gene encoding the Bacillus thuringiensis δ-endotoxin (insect resistance) 105 in-house pat Phosphinotricin-N-acetyltransferases gene from Streptomyces viridochromogenes 109 [68] bar Phosphinotricin-N-acetyltransferases gene from Streptomyces hygroscopicus 69 [68] epsps 5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain CP4 108 [68] p35S discriminating method CRT Reverse transcriptase gene from the cauliflower mosaic virus 94 in-house