406273.fig.001a
(a)
406273.fig.001b
(b)
406273.fig.001c
(c)
406273.fig.001d
(d)
Figure 1: Fibrin accumulation in both 𝐴 𝑛 𝑥 𝐴 2 / and hyperhomocysteinemic mice. Kidney sections from 𝐴 𝑛 𝑥 𝐴 2 + / + mice on normal chow (a and b), from 𝐴 𝑛 𝑥 𝐴 2 / mice on normal chow (c), or from 𝐴 𝑛 𝑥 𝐴 2 + / + mice on a high methionine diet (d). Sections from heparin-treated, highly perfused animals were stained with a polyclonal rabbit antifibrin(ogen)-specific antibody using either immunoperoxidase plus diaminobenzidine (brown immunoreaction product) with hematoxylin counterstaining [5] (a and c), or biotinylated goat anti-rabbit IgG followed by Alexa 568-conjugated streptavidin (b and d); red staining represents Alexa 568, blue represents DAPI nuclear staining, and green is tissue autofluorescence [6].