Research Article

Comparative Characterisation of Genotypically Different Clones of MRSA in the Production of Biofilms

Table 1

Relationships among the presence of icaADBC, fnbA, fnbB, fib, can, eno, ebpS, bbp, clfA, clfB, slime production, adherence capacity, and biofilm production in 30 different clinical types of Staphylococcus aureus isolates naming MRSA.

MLST
No.Spa types ST CCSCC mecBiofilm formation on MCRA a Adherence capacityBiofilm production using SEMDetermined biofim and adhesion genes *Source of samples
by PCR icaADBC fnbA fnbB fib
cnaenoebpSbbpclfAclfB

1t932ST-239 CC8 IIIABlack + producer+++++++++ b CSF
2t037ST-239CC8IIIAVery black++++Producer+++++++++pus
3t037ST-239CC8IIIABlack++++Producer+++++++++Pus
4t037 ST-1283CC8IIIAWeak black++++producer+++++++++Wound
5t421 ST- 239CC8IIIABlack++++Producer+++++++++Wound
6t421 ST 239CC8IIIAVery black++++Producer+++++++++Pus
7t421 ST- 239CC8IIIABlack++++Producer+++++++++Sputum
8t4150ST-239CC8IIIABlack+++Producer+++++++++Wound
9t4150ST-239CC8IIIABlack+++Producer+++++++++Blood
10t4150ST-239CC8IIIABlack+++Producer+++++++++Sputum
11t2575ST-239CC8IIIABlack++Producer+++++++++Abscess
12t3213 ST-22 CC22IVhBlack++Producer++++++++Blood
13t032 ST-22 CC22IVhBlack+++producer+++++++Blood
14t032 ST-22 CC22IVhBlack+++Producer+++++++Blood
15t032 ST-22 CC22IVhVery black+++Producer+++++++Pus
16t138 ST-1283CC8IIIAVery black+++Producer+++++++++Medical devices
17t138 ST-1283CC8 IIIABlack+++Producer+++++++++Blood
18t4184ST-22 CC22IVhRed+producer++++++++Blood
19t127ST-1CC1VBlack++++Producer++++++++Wound
20t127ST-1CC1VBlack++++Producer++++++++Pus
21t127 ST-1CC1VBlack++++Producer++++++++Pus
22t189 ST-188CC1VVery black++Producer++++++++Wound
23t189 ST-188CC1VBlack++Producer++++++++pus
24t189 ST-188CC1VBlack++Producer++++++++Pus
25t4213 ST-239CC8 IIIARed+++Producer+++++++++Wound
26t4213 ST-239CC8 IIIAVery black+++Producer+++++++++Sputum
27t4213 ST-239CC8 IIIABlack+++Producer+++++++++Pus
28t091 ST-7CC7VBlack++Producer++++++++Blood
29t091 ST-7CC7VVery black++Producer+++++++++Pus
30t091 ST-7CC7VBlack++Producer++++++++Pus
ATCC35556Very black+++Producer +++++++ATCC
ATCC12228RedNon-ProducerATCC

*All MRSA isolates previously were collected from Kuala Lumpur General Hospital (see Section 2.1. bacterial isolates). a : Regards the different capabilities of strains adherent on the polystyrene surface, where ++++ represents highly adherent (A490 > 3.0), +++ represents strongly adherent (A490 > 2.0), + + represents moderately adherent (A490 > 1.0–2.0), + represents weakly adherent (A490 > 0.5–1.0), and−represents non-adherent (A490 ≤ 0.5). b : Regarding the cerebro spinal fluid.