Comparative Characterisation of Genotypically Different Clones of MRSA in the Production of Biofilms
Table 1
Relationships among the presence of icaADBC, fnbA, fnbB, fib, can, eno, ebpS, bbp, clfA, clfB, slime production, adherence capacity, and biofilm production in 30 different clinical types of Staphylococcus aureus isolates naming MRSA.
MLST
No.
Spa types
ST
CC
SCC mec
Biofilm formation on MCRA
Adherence capacity
Biofilm production using SEM
Determined biofim and adhesion genes
*Source of samples
by PCR icaADBC fnbA fnbB fib
cnaenoebpSbbpclfAclfB
1
t932
ST-239
CC8
IIIA
Black
+
producer
+
+
+
+
+
+
+
−
+
+
CSF
2
t037
ST-239
CC8
IIIA
Very black
++++
Producer
+
+
+
+
+
+
+
−
+
+
pus
3
t037
ST-239
CC8
IIIA
Black
++++
Producer
+
+
+
+
+
+
+
−
+
+
Pus
4
t037
ST-1283
CC8
IIIA
Weak black
++++
producer
+
+
+
+
+
+
+
−
+
+
Wound
5
t421
ST- 239
CC8
IIIA
Black
++++
Producer
+
+
+
+
+
+
+
−
+
+
Wound
6
t421
ST 239
CC8
IIIA
Very black
++++
Producer
+
+
+
+
+
+
+
−
+
+
Pus
7
t421
ST- 239
CC8
IIIA
Black
++++
Producer
+
+
+
+
+
+
+
−
+
+
Sputum
8
t4150
ST-239
CC8
IIIA
Black
+++
Producer
+
+
+
+
+
+
+
−
+
+
Wound
9
t4150
ST-239
CC8
IIIA
Black
+++
Producer
+
+
+
+
+
+
+
−
+
+
Blood
10
t4150
ST-239
CC8
IIIA
Black
+++
Producer
+
+
+
+
+
+
+
−
+
+
Sputum
11
t2575
ST-239
CC8
IIIA
Black
++
Producer
+
+
+
+
+
+
+
−
+
+
Abscess
12
t3213
ST-22
CC22
IVh
Black
++
Producer
+
+
−
+
+
+
+
−
+
+
Blood
13
t032
ST-22
CC22
IVh
Black
+++
producer
+
+
−
−
+
+
+
−
+
+
Blood
14
t032
ST-22
CC22
IVh
Black
+++
Producer
+
+
−
−
+
+
+
−
+
+
Blood
15
t032
ST-22
CC22
IVh
Very black
+++
Producer
+
+
−
−
+
+
+
−
+
+
Pus
16
t138
ST-1283
CC8
IIIA
Very black
+++
Producer
+
+
+
+
+
+
+
−
+
+
Medical devices
17
t138
ST-1283
CC8
IIIA
Black
+++
Producer
+
+
+
+
+
+
+
−
+
+
Blood
18
t4184
ST-22
CC22
IVh
Red
+
producer
+
+
−
+
+
+
+
−
+
+
Blood
19
t127
ST-1
CC1
V
Black
++++
Producer
+
+
−
+
+
+
+
−
+
+
Wound
20
t127
ST-1
CC1
V
Black
++++
Producer
+
+
−
+
+
+
+
−
+
+
Pus
21
t127
ST-1
CC1
V
Black
++++
Producer
+
+
−
+
+
+
+
−
+
+
Pus
22
t189
ST-188
CC1
V
Very black
++
Producer
+
+
−
+
+
+
+
−
+
+
Wound
23
t189
ST-188
CC1
V
Black
++
Producer
+
+
−
+
+
+
+
−
+
+
pus
24
t189
ST-188
CC1
V
Black
++
Producer
+
+
−
+
+
+
+
−
+
+
Pus
25
t4213
ST-239
CC8
IIIA
Red
+++
Producer
+
+
+
+
+
+
+
−
+
+
Wound
26
t4213
ST-239
CC8
IIIA
Very black
+++
Producer
+
+
+
+
+
+
+
−
+
+
Sputum
27
t4213
ST-239
CC8
IIIA
Black
+++
Producer
+
+
+
+
+
+
+
−
+
+
Pus
28
t091
ST-7
CC7
V
Black
++
Producer
+
+
−
+
−
+
+
+
+
+
Blood
29
t091
ST-7
CC7
V
Very black
++
Producer
+
+
−
+
+
+
+
+
+
+
Pus
30
t091
ST-7
CC7
V
Black
++
Producer
+
+
−
+
−
+
+
+
+
+
Pus
ATCC35556
Very black
+++
Producer
+
+
−
+
−
+
+
−
+
+
ATCC
ATCC12228
Red
−
Non-Producer
−
−
−
−
−
−
−
−
−
−
ATCC
*All MRSA isolates previously were collected from Kuala Lumpur General Hospital (see Section 2.1. bacterial isolates). : Regards the different capabilities of strains adherent on the polystyrene surface, where ++++ represents highly adherent (A490 > 3.0), +++ represents strongly adherent (A490 > 2.0), represents moderately adherent (A490 > 1.0–2.0), + represents weakly adherent (A490 > 0.5–1.0), and−represents non-adherent (A490 ≤ 0.5). : Regarding the cerebro spinal fluid.