417675.fig.003a
(a)
417675.fig.003b
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417675.fig.003c
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417675.fig.003d
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417675.fig.003e
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417675.fig.003f
(f)
Figure 3: Representative photomicrographs of double immunofluorescence staining for C3 (FITC; green) and NAPlr (Alexa Fluor 594; red) ((a)–(c)). The distributions of C3 (a) and NAPlr (b) were obviously different in the merged image (c). ((d)–(f)). NAPlr IF staining and in situ zymography for plasmin activity in serial sections of renal biopsy tissue from an APSGN patient. The distribution of plasmin activity was similar to that of NAPlr deposition ((d) and (e)). Addition of aprotinin inhibited the zymographic activity suggesting the activity to be plasmin (f) (original magnification ×200).