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Figure 5: Glomerular infiltrating neutrophils and plasmin activity in APSGN and rapidly progressive glomerulonephritis. Representative photomicrographs of double staining for neutrophil elastase ((a) and (d), indirect immunofluorescence staining) and plasmin activity ((b) and (e), in situ zymography) from a patient with APSGN ((a)–(c)) and with rapidly progressive glomerulonephritis ((d)–(f)). The same fields were observed under fluorescence microscopy ((a) and (d)) and light microscopy ((b) and (e)) and were merged ((c) and (f)). The merged image (c) shows upregulated plasmin activity in a large portion of glomerular neutrophils in APSGN patients (indicated by arrows) but not in rapidly progressive glomerulonephritis patients (f) (original magnification ×260).