Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine

Figure 1

Genome maps of Towne-varS, Towne-varL, and BAC-cloned viruses. Inverted repeats 𝑏/𝑏^ and 𝑐/𝑐^ (grey boxes) flank unique long (U_L) and unique short (U_S) regions to delineate long and short genome segments. One or more copies of the a sequence (black bars) can be found at long segment termini and inverted at the long and short segment junction (𝑏^𝑎^𝑐^), while one or no copies are found at short segment termini [9, 10] (for simplicity single copies are shown). Dashed lines indicate U_L sequences in varL that are missing from varS; black boxes represent the BAC ori cassette (BAC). Nucleotide positions are indicated; genome features are approximately to scale.