Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation
Figure 3
Effects of FTH1 overexpression on cell proliferation, cytotoxicity, apoptosis, and migration. (a) High expression of FTH1 reduced cell growth without iron supplementation while did not reduce cell growth with iron supplementation. MTT assay was used to assess the effect of FTH1 overexpression on the cell proliferation. (b) FTH1 overexpression did not increase the release of G6PD regardless of Fe supplementation. High G6PD release represents increased toxicity. (c) FTH1 overexpression did not increase the cell apoptosis by hoechst apoptosis assay regardless of iron supplementation. (d) High expression of FTH1 reduced cell migration without iron supplementation while did not reduce cell migration with iron supplementation. Data were normalized by those in B-7 cells in 10 ng/mL Dox which was defined as 100%; G6PD, glucose-6-phosphate dehydrogenase. Data were expressed as mean ± SD from 6 experiments. (*: two-tailed unpaired -test.)
