Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation
Figure 5
B-7 cells under both derepressed state (FTH1 overexpression) and the presence of FAC supplementation was capable of producing visible contrast by MRI. (a) B-7 cells were cultured with or without 10 ng/mL doxycycline and with or without FAC for -weighted images. Arrow indicated the sample with the highest , in the presence of both FTH1 overexpression and iron supplementation (200 μM) for 48 h. (b) B-7 cells were grown for 48 h in the presence of FAC at different concentrations. (c) B-7 cells grown in doxycycline (Dox) at different concentrations for 96 h, and in 200 μM FAC for 48 h before analysis. Relaxation rates derived from the maps were expressed as mean ± SD for 3 experiments.
