Artificial Chromosomes to Explore and to Exploit Biosynthetic Capabilities of Actinomycetes

Figure 2

Generalized workflow for heterologous production of natural compounds in an amenable host. A high molecular weight genomic library is constructed and screened to identify one or two clones containing the biosynthetic gene cluster of interest. Once the clone is identified and sequenced to confirm the presence of all the genes, the recombinant vector DNA is used to transform the amenable host. Last, the heterologous production can be improved by changing fermentation process or by genetically manipulating the host.