Artificial Chromosomes to Explore and to Exploit Biosynthetic Capabilities of Actinomycetes

Table 2

Actinomycetes genomic libraries constructed in artificial chromosomes.

Source	Genome coverage	Average insert size	Genomic DNA digested with	Vector	Reference
Planobispora rosea ATCC 53773	5X	60 kb	Sau3AI	pPAC-S2	[28]
Nonomuraea sp. ATCC 3972	5X	57 kb	Sau3AI	pPAC-S2	[29]
S. roseosporus NRRL 11379	17X	71 kb	BamHI	pSTREPTOBAC V	[22]
S. platensis NRRL18993	22X	75 kb	BamHI	pSTREPTOBAC V	[31]
Streptomyces sp. NRRL 30748	NR	NR	MfeI	pSBAC^*	[23]
S. autolyticus JX-47	50X	150 kb	Sau3AI	pIndigoBAC5^*	[32]

NR: not reported.
^*Not integrative in Streptomyces.