Research Article

New Protein Vector ApE1 for Targeted Delivery of Anticancer Drugs

Table 1

Yield of the target protein ApE1 in successive purification stages.

StagesApE1* output (from 1 L of culture medium), mgYield, %

Biomass40100
Soluble fraction3280
1st purification step (DEAE-Sepharose CL6B)3075
2nd purification step (Chelating Sepharose FF)2870
Purified ApE12870

*The target protein amounts were determined using the ImageJ program after SDSPAGE.