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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 482568, 10 pages
http://dx.doi.org/10.1155/2012/482568
Research Article

Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library

1Department of Animal Science & Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea
2Research and Analysis Division, National Agricultural Products Quality Management Service, Gyungki 430-824, Republic of Korea
3Subtropical Animal Experiment Station, National Institute of Animal Science, Jeju 690-150, Republic of Korea
4Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea

Received 27 April 2011; Revised 8 June 2011; Accepted 16 June 2011

Academic Editor: Guihua H. Bai

Copyright © 2012 Seong-Lan Yu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pigs have been considered as donors for xenotransplantation in the replacement of human organs and tissues. However, porcine endogenous retroviruses (PERVs) might transmit new infectious disease to humans during xenotransplantation. To investigate PERV integration sites, 45 PERV-positive BAC clones, including 12 PERV-A, 16 PERV-B, and 17 PERV-C clones, were identified from the NIH miniature pig BAC library. The analysis of 12 selected full-length sequences of PERVs, including the long terminal repeat (LTR) region, identified the expected of open reading frame length, an indicative of active PERV, in all five PERV-C clones and one of the four PERV-B clones. Premature stop codons were observed in only three PERV-A clones. Also, eleven PERV integration sites were mapped using a 5000-rad IMpRH panel. The map locations of PERV-C clones have not been reported before, thus they are novel PERV clones identified in this study. The results could provide basic information for the elimination of site-specific PERVs in selection of pigs for xenotransplantation.