Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library

Table 2

PCR conditions and primer information for contig mapping of PERV positive clones.

Type of PERV	BAC clone ID		Primer sequence (5′→3′)		Annealing temperature (°C)	PCR product (bp)
	A1-1F	T7	F: CCTGAGCGTGAAAAGATTCC	R: GGCCACTTGATTATGGCAGT	56	298
		SP6	F:CCATGTAGAAGAGACACCTGTTG	R:TGAAGGTCCTGAGGTGTATGG	56	290
	A1-1E	T7	F:CCTGAGCGTGAAAAGATTCC	R:GGCCACTTGATTATGGCAGT	56	298
		SP6	F:CCATGTAGAAGAGACACCTGTTG	R:TGAAGGTCCTGAGGTGTATGG	56	290
	A1-2G	T7	F: CCTGAGCGTGAAAAGATTCC	R: GGCCACTTGATTATGGCAGT	56	298
		SP6	F:CCATGTAGAAGAGACACCTGTTG	R: TGAAGGTCCTGAGGTGTATGG	56	290
	A1-6C	T7	F: CCAATGATTCACTGACCTTTGA	R: TCCCTAGAGGGCTTCCTTTC	56	214
		SP6	F: ACGTGGAGACGTGAAGAACC	R: GACTCGAATCCAGCCTAAACC	56	111
A	A1-6E	T7	F: TAGCATTTCCATCCCAAACC	R:TTGCAGAGCAGATACTGACTCAC	56	105
		SP6	F: AGGTGGAAGCTACCTCAGCA	R: GCAGAGCTCTTACGCAGTCA	63	186
	A3-9F	SP6	F: TCATGTGCAGTTCATGGAAG	R: TGGGAGTGGAGATTGTCCTT	56	183
	A3-10E	SP6	F: TCATGTGCAGTTCATGGAAG	R: TGGGAGTGGAGATTGTCCTT	56	183
	A4-1G	T7	F: CTACGAAGGGGTCTGAGCAC	R: TGGGCAGGTATTGAGGTTTC	56	174
		SP6	F: CTGTCAGTGTGAAGGGCTGA	R: CTCTCGGCTTCTTCCTCCTT	63	248
	A4-1H	T7	F: CATTGTTTGTCCAGTGCTGTG	R: ATACCTGGCTTGGCACAGAG	56	163
		SP6	F: AGGCTCATTTTCATGCCATT	R: GCCCACATTTTGTTTCTATGG	56	247
	A4-14H	T7	F: TGAAGACCAATGTCCTGTGG	R: TGCCTTCAATTAAGCCCTCA	63	229
	B1-7A	T7	F: TCAAAGTCCTCCCCAGTGTT	R: CATTGACTAGGGCAGGGGTA	56	180
	B1-8D	T7	F: AGCTCACCAGGAAAGGCATA	R: TCTCCAGTATCCTGCGTTTG	56	111
		SP6	F: AATTCTCCCCTGGTTTCACC	R: TGCAGTTGGAGGGAGCTAAT	56	126
	B1-11C	T7	F: TTGGGTGTATTTTGTGGCTTC	R: GCAACCAGTTCAAGGCAGAG	56	282
	B2-10D	SP6	F: GTTTCTGAGGCCAAAACAGC	R: GGCACCCTAGAGTTTTGCAG	56	154
	B2-11A	SP6	F: GTTTCTGAGGCCAAAACAGC	R: GGCACCCTAGAGTTTTGCAG	56	154
	B3-3G	T7	F: AAAGCTCTATCCTTTGGAGACAGA	R: TACTGGTGGCATGGTTTTGA	56	211
	B3-7F	T7	F: CAGCCGTTGCAGAAATCTTA	R: GCAAGGTAAGCCAAGGATTG	60	182
B		SP6	F: CTGCAGGGAAAATAGATGGTT	R: GACCATGGAGAAGAAAGGTTG	56	259
	B3-7G	T7	F: TCCAAGCTTAGAAGGATGCAA	R: GCTCATGCTAGCAAAGCCTTA	60	244
		SP6	F: GAGCAACGTGCTAACAAAACTG	R: TTTGTTATGACAATGTGGTTTCA	56	150
	B3-11C	T7	F: CCACAGCTAACGGAGTGGTT	R: AAAGAAACAGGCAGGGGTTT	56	204
	B3-12E	SP6	F: TGTGCCCAACTGCTACTGTC	R: TACCCTCATGGGCAATTCTC	60	263
	B3-12H	T7	F: CTGCAACAGAATGGCATAAGA	R: GCACTCGATGTGCTTTTTCA	60	138
	B4-1B	T7	F: CCCACTGGAAAAAGGAAAGA	R: ATTAGCGGGTGCCCTTCTAT	56	199
	B4-2E	SP6	F: ACTCCCAGGGAATTCAGGTT	R: TGTGGTATTCAAGGCAGGAA	56	158
	B4-13H	T7	F: GTTTCTGAGGCCAAAACAGC	R: GCCACCCTAGAGTTTTGCAG	56	156
		SP6	F: AGAGAAGGAAGGGGAGGACA	R: CTGCTTCCTCCCAGTCTCAG	56	108
	C1-1D	T7	F: TCTAGGCCCAATTGGTTTTG	R: TCACTTAGCCCGACCCATAG	63	200
		SP6	F: TCATCTAAATGCTTGCTGTTTG	R: AAATGCCTGTGCCTTTTGAG	63	281
	C1-9B	T7	F: ATGGAGCTGACAAACCCTTG	R: CAATCACAAGCCACATGTCC	63	197
	C1-9D	T7	F: ATGGAGCTGACAAACCCTTG	R: CAATCACAAGCCACATGTCC	63	197
	C1-10G	T7	F: CTCTGGTCCTGGGCAAATAC	R: CAGCCTTTCCATCACTCCAC	63	214
		SP6	F: TGTATCCAGGAGGCATCACA	R: TTGGTCTGCATTGAGGACTG	63	194
	C2-3F	SP6	F: CAGCAGTGGTCCTGTTGAGA	R: CCAGCCTTTGTCACTCTTCAC	63	265
C	C2-5F	T7	F: TTTCGTTGCTCTCACCTTGA	R: GGTGGAGTGGAGTTTGTGGA	63	205
	C2-6C	SP6	F: TGCAATAGAGCAAAGGGAGAA	R: AACCTGACCTTCCCCAAGAT	63	268
	C2-6H	SP6	F: GGAAGATTTTTGGATACGTGTG	R: TGAGTTTTCCAGTTGCATGG	60	163
	C3-1E	T7	F: CTCTGAGGACACAGGCTTCC	R: ACTGCCACTCCAACCACTCT	60	150
		SP6	F: CAGAACCCGTCCACTGAAAT	R: TCCAGGGAGAAAACTGGTTG	63	196
	C3-4B	T7	F: CTCTGAGGACACAGGCTTCC	R: ACTGCCACTCCAACCACTCT	60	150
		SP6	F: CAGAACCCGTCCACTGAAAT	R: TCCAGGGAGAAAACTGGTTG	63	196
	C3-6F	T7	F: CGTGTTTGAGGAGCACACAT	R: GTCCACAGAAGCCAGAGAGG	63	211
	C4-2G	T7	F: ATAATCACAATCTGTGGGCATA	R: GCTCAACCCCAGACATTTAGAC	60	108
		SP6	F: TGCTGTTCTTTACTGGGCATT	R: ATCCCAGCTGCCAAATACAC	63	265