Artificial Neural Network for the Prediction of Tyrosine-Based Sorting Signal Recognition by Adaptor Complexes
Figure 4
Binding of Cd63-Lamp2 chimeric Y-signals to . (a) Two-hybrid experiments between subunit and WT or chimeric Y-signals were performed as described in Figure 1 and Section 2.4. Cd63-specific and Lamp2-specific residues are denoted in blue and red, respectively. (b) HeLa cells transiently transfected with TAC-fusion proteins were fixed, permeabilized, and incubated with mouse anti-TAC antibody followed by an Alexa 448-conjugated secondary antibody. Representative cells showing TAC fusion protein localization are shown. Arrows (left panel) point to intracellular structures observed in TAC-HTGYEQF (Lamp2 signal) fusion; Arrowheads (right panel) highlight plasma membrane enrichment of TAC-HTGYEMV chimeric signal fusion protein. Scale bar: 1 μm.
